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Dendritic Cell Marker DCIR2 Rat anti-Mouse, Clone: 33D1, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$63.00 - $258.00

Specifications

Antigen Dendritic Cell Marker DCIR2
Clone 33D1
Target Species Mouse
Conjugate Unconjugated
Isotype IgG2b, kappa
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-132-70 Non-distribution product as customer accommodation.
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Invitrogen
14588481
50 μg Each for $63.00
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50-132-72 Non-distribution product as customer accommodation.
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Invitrogen
14588485
500 μg Each for $258.00
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50-132-71 Non-distribution product as customer accommodation.
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Invitrogen
14588482
100 μg Each for $96.25
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Description

Description

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer ft.s patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii. Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining. It has also been reported in complement-mediated cytotoxicity. Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For detection of purified 33D1 it is recommended not to use goat anti-rat due to its low affinity for 33D1. Rather, we recommend using biotin mouse anti-rat (cat. 13-4813).

Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer ft.s patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
Specifications

Specifications

Dendritic Cell Marker DCIR2
Mouse
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C
33D1
Unconjugated
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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