Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-588-481	Invitrogen 14588481	50 μg
50-132-71	Invitrogen 14588482	100 μg

Description

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii. Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining. It has also been reported in complement-mediated cytotoxicity. Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For detection of purified 33D1 it is recommended not to use goat anti-rat due to its low affinity for 33D1. Rather, we recommend using biotin mouse anti-rat (cat. 13-4813).

The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

Specifications

• Antigen: Dendritic Cell Marker DCIR2
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene ID (Entrez): 474145
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 33D1
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Mouse
• Gene Symbols: Clec4a4
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: Clec4a4

For Research Use Only.