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Thermo Scientific Chemicals Deoxyribonuclease I, bovine pancreas, Purified

Catalog No. AAJ61061LB0
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Description

Deoxyribonuclease I, CAS # 9003-98-9, also known as DNase I, is an endonuclease isolated from bovine pancreas used mainly to remove deoxyribonucleic acid (DNA) from protein samples.

Deoxyribonuclease I, is used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. It is used in investigating the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • Deoxyribonuclease I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5'-phosphates. In the presence of Mg2+, this compound cleaves each strand of DNA independently

Application

  • In biomedical research, this compound is used to nick DNA as a first step to integrate labeled bases into DNA
  • It can be used to eliminate genomic DNA from RNA solutions before RT-PCR
  • It is also used during isolation and purification of plasma membrane vesicles from Neurospora crassa cell culture
  • It can decrease viscosity in cell culture lysates, providing better yields in primary cell isolation
  • It is also commonly used in combination with trypsin for the preparation of single cell suspension from rats
  • Deoxyribonuclease I, bovine pancreas has been used in some studies in order to compare different methods for reducing RNase contamination in preparations of DNase
  • This compound can be applied in studies to investigate the impact of the composition of sodium dodecyl sulfate (SDS) preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis
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Specifications

Quantity 5 mg
Form Lyophilized

RUO – Research Use Only

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