Invitrogen™ Desmin Monoclonal Antibody (DE-U-10), Alexa Fluor™ Plus 750

Catalog No.	Quantity
75-797-4794	500 μL

Catalog No. 75-797-4794 Supplier Invitrogen™ Supplier No. 757974794

Description

Mouse Monoclonal Antibody

Description: The monoclonal antibody DE-U-10 recognizes desmin. Desmin is the protein subunit of class-III intermediate filaments. Desmin is found predominantly in skeletal, cardiac, and smooth muscle. Desmin plays a role in muscle cell development and differentiation, muscle cell architecture and structure, and mitochondrial function. Desmin forms scaffolds around the Z-disk of sarcomeres in muscle cells. Desmin knockout mice exhibit defects in skeletal, smooth, and cardiac muscle and have impaired mitochondrial function. Mutations in Desmin result in conditions such as desmin-related myopathy (DRM), cardiomyopathy dilated type 1I (CMD1I), and neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome). The DE-U-10 antibody recognizes human, mouse, rat, feline, bovine, goat, hamster, sheep, porcine, rabbit, and chicken desmin. The DE-U-10 antibody will also label tumors derived from muscle tissue, such as leiomyomas and rhabdomyosarcomas. Applications Reported: This DE-U-10 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This DE-U-10 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 2 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Using conjug...

Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Specifications

• Antigen: Desmin
• Applications: Immunohistochemistry (Paraffin), Multiplex Immunohistochemistry
• Classification: Monoclonal
• Clone: DE-U-10
• Concentration: 0.2 mg/mL
• Conjugate: Alexa Fluor Plus 750
• Formulation: PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2
• Gene: DES
• Gene Accession No.: P17661
• Gene Alias: CMD1I; CSM1; CSM2; DES; Desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; I79_018932; intermediate filament protein; LGMD2R; MFM1; muscle-specific intermediate filament desmin; muscle-specific intermediate filament protein; mutant desmin p.K241E; SCPNK; similar to desmin
• Gene Symbols: DES
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1674
• Target Species: Human
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ