Edvotek™ Detecting COVID-19 Using Reverse-Transcription PCR (RT-PCR)

Description

In this simulated RT-PCR experiment, students will explore the diagnostic test used worldwide to diagnose and monitor the spread of COVID-19.

• This experiment requires a PCR thermal cycler
• Please note, this experiment is a DNA simulation of reverse transcription PCR and is intended solely for educational purposes. There are no viral or human DNA products included in this experiment. The experiment will not perform viral RNA testing for SARS-CoV-2
• For 25 students working in 5 groups
• Time required: Set up - 30 minutes, PCR - 2 hours or overnight, Electrophoresis - 45 minutes
• Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, DNA Templates, TE Buffer, UltraSpec-Agarose™, TBE Electrophoresis Buffer powder, SYBR™ Safe Stain, FlashBlue™ DNA Stain, Microcentrifuge & PCR Tubes
• Some components require freezer storage upon receipt

Specifications

• Product Type: PCR Kit
• Grade: 9 to Undergraduate
• Age: 15 to 20
• For Use With (Application): Explore the diagnostic test used worldwide to diagnose and monitor the spread of COVID-19.
• Includes: Instructions, PCR EdvoBeadsTM, LyphoPrimerTM Mix, EdvoQuickTM DNA Ladder, DNA Templates, TE Buffer, UltraSpec-AgaroseTM, TBE Electrophoresis Buffer powder, SYBR™ Safe Stain, FlashBlueTM DNA Stain, Microcentrifuge & PCR Tubes
• Requires: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 μL, UV or Blue Light Transilluminator, White Light Box (optional - use if staining with FlashBlueTM), Microcentrifuge & Microwave or Hot plate.
• Content And Storage: Some Components Require Freezer Storage Upon Receipt
• Class Size: 25 Students (5 Groups)

**Please note, this experiment is a DNA simulation of reverse transcription PCR and is intended solely for educational purposes. There are no viral or human DNA products included in this experiment. The experiment will not perform viral RNA testing for SARS-CoV-2.**