Invitrogen™ Dicer Monoclonal Antibody (N167/7), PerCP

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545570

Catalog No. PIMA545570

Description

1 μg/mL of MA5-45570 was sufficient for detection of Dicer in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 215kDa. This antibody was formerly sold as clone S167-7.

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

Specifications

• Antigen: Dicer
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: Q8R418, Q9UPY3
• Gene Symbols: DICER1
• Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot, Immunocytochemistry
• Clone: N167/7
• Conjugate: PerCP
• Gene: DICER1
• Gene Alias: 1110006F08Rik; D12Ertd7e; DCR; DCR1; DCR-1; Dicer; dicer 1 ribonuclease III; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease III; dicer 1, ribonuclease type III; Dicer1; Dicer1, Dcr-1 homolog; Dicer1e; double-strand-specific ribonuclease mDCR-1; endoribonuclease Dicer; Helicase MOI; helicase with RNAse motif; helicase-moi; HERNA; K12H4.8-LIKE; KIAA0928; mKIAA0928; MNG1; RMSE2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 192119, 23405, 299284
• Content And Storage: 4°C
• Form: Liquid