Invitrogen™ DIO1 Polyclonal Antibody

Catalog No.	Quantity
PIPA5143925	100 μg

Catalog No. PIPA5143925 Supplier Invitrogen™ Supplier No. PA5143925

Description

Rabbit Polyclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: rat liver tissue, rat kidney tissue, human A549 whole cell. ICC/IF: A549 cell. Flow: 293T cell, ANA-1 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some, but not all, isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence.

Specifications

• Antigen: DIO1
• Applications: ELISA, Flow Cytometry, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene: Dio1
• Gene Accession No.: P24389, P49895, Q61153
• Gene Alias: 5DI; D1; deiodinase, iodothyronine type I; deiodinase, iodothyronine, type I; Dio1; DIOI; hDido1; iodothyronine deiodinase type 1; ITDI1; thyroxine 5'-deiodinase; thyroxine deiodinase; thyroxine deiodinase type I (selenoprotein); thyroxine deiodinase, type I; TXDI1; Type 1 DI; type I iodothyronine deiodinase; Type-I 5'-deiodinase
• Gene Symbols: Dio1
• Host Species: Rabbit
• Immunogen: E.coli-derived human DIO1 recombinant protein (Position: K137-S249).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13370, 1733, 25430
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG