Novus Biologicals™ Dkk-1 Antibody Pair [HRP]

Catalog No.	Quantity
NB010371	15 Plates

Catalog No. NB010371 Supplier Novus Biologicals™ Supplier No. NBP27966615PLATES

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Dkk-1.

Specifications

• Assay Range: 39.06 pg/mL
• Assay Sensitivity: 39.06-2500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Dkk-1.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, DKK-1, hDkk-1, SKdickkopf-1 like
• Gene ID (Entrez): 22943
• Gene Symbol: DKK1
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 20 ng of recombinant Human Dkk-1.
  Capture Antibody: 0.5 mg/mL of mouse anti-Human Dkk-1 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of mouse anti-Human Dkk-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Immunology, Innate Immunity, Signal Transduction, Stem Cell Signaling Pathway, Wnt Signaling Pathway
• Storage Requirements: Storage is content dependent.
• Target: Dkk-1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.