Novus Biologicals™ Dkk-3 Antibody Pair [HRP]

Catalog No.	Quantity
NB010137	15 Plates

Catalog No. NB010137 Supplier Novus Biologicals™ Supplier No. NBP27954915PLATES

Description

Polyclonal/Monoclonal Antibody Pair

specifically detects in Mouse samples. It is validated for ELISA, Sandwich ELISA.

Specifications

• Assay Range: 15.625 pg/mL
• Assay Sensitivity: 15.63-1000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Mouse Dkk-3.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: dickkopf (Xenopus laevis) homolog 3, dickkopf 3, dickkopf homolog 3 (Xenopus laevis), Dickkopf-3, dickkopf-related protein 3, Dkk-3, hDkk-3, regulated in glioma, REICdkk-3, RIG, RIG-like 5-6, RIG-like 7-1
• Gene ID (Entrez): 27122
• Gene Symbol: DKK3
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.5 mg/mL rabbit anti-Dkk-3 polyclonal antibody conjugated to horseradish-peroxidase (HRP).
  Capture Antibody: 0.5 mg/mL of rabbit anti-mouse Dkk-3 monoclonal antibody.
  Standard: Each vial contains 15 ng of recombinant mouse Dkk-3.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Signal Transduction
• Storage Requirements: Storage is content dependent.
• Target: Dkk-3
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2.0 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute standard powder with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.