Invitrogen™ DKK1 Recombinant Rabbit Monoclonal Antibody (SC06-86)

Catalog No.	Quantity
PIMA532229	100 μL

Catalog No. PIMA532229 Supplier Invitrogen™ Supplier No. MA532229

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.

Specifications

• Antigen: DKK1
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Western Blot
• Classification: Recombinant Monoclonal
• Clone: SC06-86
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene: DKK1
• Gene Accession No.: O94907
• Gene Alias: dickkopf 1 homolog; dickkopf homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; dickkopf WNT signaling pathway inhibitor 1; Dickkopf1; dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; dickkopf-related protein 1; DKK1; DKK-1; hDkk1; hDkk-1; mdkk-1; SK; UNQ492/PRO1008
• Gene Symbols: DKK1
• Host Species: Rabbit
• Immunogen: Synthetic peptide within Human DKK1 aa 225-266.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 22943, 293897
• Target Species: Human, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG