DLL4 (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P279BT25	Invitrogen™ 500P279BT25UG	25 μg
500P279BT50	Invitrogen™ 500P279BT50UG	50 μg
50-271-8542	Invitrogen™ 500P279BT500UG	500 μg

Description

AA Sequence of recombinant protein: SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sDLL-4. Anti-Human sDLL-4-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hsDLL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sDLL-4 (500-P279) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hsDLL-4. Western Blot: To detect hsDLL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsDLL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P279BT-1MG will be provided as 2 x 500 μg

Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

Specifications

• Antigen: DLL4 (soluble)
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q9NR61
• Gene ID (Entrez): 54567
• Immunogen: HEK293 cells-derived Recombinant Human sDLL-4.
• Purification Method: Antigen Affinity Chromatography
• Gene: Dll4

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: AOS6; delta 4; delta ligand 4; delta like canonical Notch ligand 4; Delta4; delta-like 4 (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4; UNQ1895/PRO4341
• Gene Symbols: Dll4
• Primary or Secondary: Primary
• Content And Storage: -20°C