Invitrogen™ DM4 Recombinant Rabbit Monoclonal Antibody (3H3)

Catalog No.	Quantity
PIMA544657	100 μL

Catalog No. PIMA544657 Supplier Invitrogen™ Supplier No. MA544657

Description

Rabbit Recombinant Monoclonal Antibody

Application notes: Indirect ELISA.

Ravtansine (DM4) is a maytansinoid, a chemical derivative of maytansine being investigated as the cytotoxic payload of a number of antibody-drug conjugates (ADCs). Microtubules are dynamic cytoskeletal polymers that switch stochastically between states of growing and shortening, called dynamic instability. They function in the precise segregation of chromosomes during cell division, transport of cellular cargos, and positioning and movement of intracellular organelles. Inhibition of microtubule function leads to cell cycle arrest and cell death. Microtubule-targeted drugs including the Vinca alkaloids, taxanes, and epothilones suppress the dynamic instability of microtubules, induce mitotic arrest, inhibit cell proliferation and induce apoptosis. The anticancer properties of maytansinoids have been attributed to their ability to disrupt microtubule function. The maytansinoid emtansine (DM1), for example, binds at the ends of microtubules and thereby suppress their dynamic instability. It is synthesized in order to link maytansinoids to antibodies via disulfide bonds. Maytansinoids inhibit tubulin polymerization and microtubule assembly and enhance microtubule destabilization, so there is potent suppression of microtubule dynamics resulting in a mitotic block and subsequent apoptotic cell death. DM4 can be used in the preparation of antibody drug conjugate. Although S-methyl DM1 and S-methyl DM4 inhibited microtubule assembly more weakly than maytansine, they suppressed dynamic instability more strongly than maytansine. Like vinblastine, the maytansinoids potently suppress microtubule dynamic instability by binding to a small number of high affinity sites, most likely at microtubule ends. Thus, the maytansine derivatives that result from cellular metabolism of the antibody conjugates are themselves potent microtubule poisons, interacting with microtubules as effectively as or more effectively than the parent molecule.

Specifications

• Antigen: DM4
• Applications: ELISA
• Classification: Recombinant Monoclonal
• Clone: 3H3
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Alias: Ravtansine
• Host Species: Rabbit
• Immunogen: DM4 coupled with OVA.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG
• Target Molecule: Chemical