Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Supplier:  Thermo Scientific™ EN0523

Catalog No. FEREN0523

Description

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions.

• In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion
• In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides

Highlights

• Recombinant enzyme
• Purified from non-animal host with a lower level of intrinsic RNases

Applications

• Preparation of DNA-free RNA
• Removal of template DNA followingin vitrotranscription
• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
• DNA labeling by nick-translation in conjunction with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting
• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifications

• Concentration: 50 U/μL
• Compatible Buffer: Reaction Buffer
• Product Type: DNase I RNase Free

• Enzyme: DNase
• Quantity: 1,000 units

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.