Invitrogen™ DNMT3B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1884

Catalog No. PA1884

Description

PA1-884 detects DNA methyltransferase 3 from human and mouse tissues and cells as well as recombinant human Dnmt3. This antibody has been used to detect both recombinant and endogenous DNMT3b and recombinant DNMT3a. PA1-884 has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects DNMT3 from P19 nuclear extracts. The PA1-884 immunogen is a synthetic peptide corresponding to residues 1-14 of mouse DNMT3b. This sequence is 79% conserved between human and mouse. PA1-884 immunizing peptide (Cat. # PEP-117) is available for use in neutralization and control experiments.

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

Specifications

• Antigen: DNMT3B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Dnmt3b
• Gene Alias: DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase 3 beta; DNA methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA methyltransferase MmuIIIB; DNA MTase HsaIIIB; DNA MTase MmuIIIB; Dnmt3; DNMT3B; ICF; ICF1; M.HsaIIIB; m.MmuIIIB; MGC1090; MmuIIIB
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13436, 1789
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: O88509, Q9UBC3
• Gene Symbols: Dnmt3b
• Immunogen: Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG