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Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 405, Invitrogen™

Donkey Polyclonal Antibody

Supplier:  Thermo Scientific A48258

Catalog No. PIA48258

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To minimize cross-reactivity, the donkey anti-rabbit IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, chicken, guinea pig, hamster, horse, sheep, mouse, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. Specificity: This antibody binds to heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody does not bind non-immunoglobulin rabbit serum proteins or IgG from bovine, goat, chicken, guinea pig, hamster, horse, sheep, mouse, rat, or human.

Rabbit IgG (H+L) Highly Cross-Adsorbed Polyclonal antibody specifically detects Rabbit IgG (H+L) Highly Cross-Adsorbed in Rabbit samples. It is validated for Immunocytochemistry, Immunofluorescence


IgG (H+L) Highly Cross-Adsorbed
Alexa Fluor Plus 405
Affinity chromatography
Immunocytochemistry, Immunofluorescence
2 mg/mL
proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5
Gamma Immunoglobins Heavy and Light chains
1 mg
4° C, store in dark


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