Thermo Scientific™ DreamTaq™ Buffers (10X)

Catalog No. FERB71

Supplier: Thermo Scientific™ B71

Description

Thermo Scientific 10X DreamTaq Buffer is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase.

Thermo Scientific DreamTaq Buffer (10X), available in colorless and green formats, is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase. It contains both KCl and (NH₄)₂SO₄ to provide high specificity of primer annealing at a wide range of MgCl₂ concentrations. 10X DreamTaq buffer includes 20 mM MgCl₂

In addition, 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes. The density reagent allows direct loading of PCR products on a gel. The blue dye (migrates with 3–5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel) are included for monitoring electrophoresis progress. The dyes have excitation peaks at 424 nm and 615 nm.

Notes

• For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq Buffer.

Specifications

• Chemical Name or Material: DreamTaq Green Buffer
• Color: Green
• Concentration: 10X
• For Use With (Application): Hot-start PCR
• Quantity: 4 x 1.25 mL
• Formulation: Nonidet P40 Ethyl phenolpoly(ethyleneglycolether) 9016-45-9 ( <0.9 ), Polyethylene glycol 25322-68-3 ( 7-13 ), Sucrose 57-50-1 ( 10-30 )
• Includes: • 10X DreamTaq Green Buffer (with 20 mM MgCl₂), 4 x 1.25 mL
  
  Store in a non-frost-free freezer at -20°C.

For Research Use Only. Not for use in diagnostic procedures.