Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Catalog No.	Quantity
PIA39267	10 x 2 mg
PI21655	50 mg
PI21555	1 g

Catalog No. PIA39267 Supplier Thermo Scientific™ Supplier No. A39267

Description

Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.

Thermo Scientific™ Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Amine reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Membrane-permeable, allowing for intracellular crosslinking
• High purity, crystalline reagent can be used to create high-purity conjugates
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to BS3 (Sulfo-DSS)

Specifications

• Chemical Reactivity: Amine-Amine
• Cleavable: No
• Description: DSS
• Molecular Weight (g/mol): 368.35
• PEGylated: No
• Spacer Arm Length: 11.4 Å
• Content And Storage: Upon receipt store desiccated at 4°C.
• Cell Permeability: Yes
• Shipping Condition: Ambient
• Product Line: No-Weigh
• Labeling Method: Chemical Labeling
• Crosslinker Type: Homobifunctional
• Reactive Moiety: NHS Ester
• Spacer: Medium (10 to 30 Å)
• Form: Solid
• Quantity: 10 x 2 mg
• Solubility: DMF, DMSO
• Format: Standard, Single-use
• Water Soluble: No
• Product Type: Crosslinker

Frequently Asked Questions (FAQs)

What is the protocol for crosslinking IgG to Dynabeads from the Dynabeads Protein G Immunoprecipitation Kit (Cat. No. 10007D) using DSS (disuccinimidyl suberate)?

For crosslinking the antibody to Protein G Dynabeads, please refer to the crosslinking protocol with BS3 (bis(sulfosuccinimidyl)suberate) or Sulpha-DSS, which is an analogue of DSS. The only difference between these two molecules is that DSS is water insoluble and needs to be solubilized in DMSO before use. You can find the protocol at the following link.

Can you provide the shelf-life for DSS (disuccinimidyl suberate)?

DSS (disuccinimidyl suberate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

For Research Use Only. Not for use in diagnostic procedures.