Invitrogen™ DYKDDDDK Tag Monoclonal Antibody (FG4R), eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-2715	25 μg

Catalog No. 50-112-2715 Supplier Invitrogen™ Supplier No. 14668180

Description

Mouse Monoclonal Antibody

Description: This FG4R monoclonal antibody reacts with the DYKDDDDDK tag. This motif is frequently fused to the N- or C-terminus of proteins to facilitate purification (e.g., affinity chromatography and immunoprecipitation) and/or detection. This antibody recognizes the DYKDDDDK anywhere within the protein of interest (i.e., N-terminus, C-terminus, or internal). Applications Tested: This FG4R antibody has been tested by immunoblotting of reduced cell lysate expressing a DYKDDDDK-tagged protein at less than or equal to 1 μg/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

Specifications

• Antigen: DYKDDDDK Tag
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: FG4R
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Tag
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ
• Target Molecule: Tag

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.