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Invitrogen™ DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17)

Catalog No. PI701629
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100 μg
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PI701629 100 μg
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Catalog No. PI701629 Supplier Invitrogen™ Supplier No. 701629
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Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
TRUSTED_SUSTAINABILITY

Specifications

Antigen DYKDDDDK Tag
Applications Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Recombinant Monoclonal
Clone 8H8L17
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide
Gene Alias DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Host Species Rabbit
Immunogen Synthetic peptide (DYKDDDDK).
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
Target Molecule Tag
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