Novus Biologicals™ E-Cadherin Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human E-Cadherin.

Specifications

• Assay Range: 25 pg/mL
• Assay Sensitivity: 25-1600 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human E-Cadherin.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDHE, cell-CAM 120/80, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVOE-Cadherin, uvomorulin
• Gene ID (Entrez): 999
• Gene Symbol: CDH1
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 115 ng of recombinant Human E-Cadherin
  pan Cadherin monoclonal antibody (in PBS, pH 7.4).
  pan Cadherin polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Cell Cycle and Replication, Cellular Markers, Extracellular Matrix, Phospho Specific, Plasma Membrane Markers, Signal Transduction
• Storage Requirements: Storage is content dependent.
• Target: E-Cadherin
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in Dilution Buffer before use. 3. Standard: Reconstitute with 1 ml Dilution Buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 1600 pg/mL is recommended.