Invitrogen™ E-cadherin Monoclonal Antibody (4A2C7)

Catalog No.	Quantity
33-400-0	100 μg

Catalog No. 33-400-0 Supplier Invitrogen™ Supplier No. 334000

Description

Mouse Monoclonal Antibody

This antibody is specific for E-cadherin but does show some minor cross-reactivity with P-cadherin. Formalin-fixed, paraffin embedded tissue sections required a Heat Induced Epitope Retrieval (HIER) step prior to staining with this antibody in an IHC application.

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

Specifications

• Antigen: E-cadherin
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 4A2C7
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene: CDH1
• Gene Accession No.: P12830
• Gene Alias: AA960649; ARC-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell adhesion molecule; cell-CAM 120/80; ECAD; E-cad; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Ecadherin; E-cadherin; E-cadherin 1; E-cadherin precursor; Epithelial cadherin; hab; half baked; LCAM; L-CAM; Um; UVO; Uvomorulin
• Gene Symbols: CDH1
• Host Species: Mouse
• Immunogen: Recombinant MBP fusion protein corresponding to the cytoplasmic domain of human E-cadherin.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 999
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ