MilliporeSigma™ E. coli anti-pan-ADP-ribose binding reagent, Polyclonal,

Catalog No.	Quantity
MABE1016MI	200 μL

Catalog No. MABE1016MI Supplier MilliporeSigma™ Supplier No. MABE1016

Description

E. coli Polyclonal Antibody

Specifically detects pan-ADP-ribose binding reagent in Human, Mouse samples, and it is validated for Dot Blot

Anti-pan-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta™(DE3)pLysS strain of E. coli. Anti-pan-ADP-ribose binding reagent is useful for the affinity detection of both mono- and poly-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis, The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-pan-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.

Specifications

• Antigen: pan-ADP-ribose binding reagent
• Applications: Dot Blot
• Classification: Polyclonal
• Formulation: Purified from E. coli by Ni-NTA agarose. Supplied in buffer containing 10mM Tris pH 7.5, 0.2M NaCl, 10% Glycerol, 10mM Imidazole, 1mM PMSF, 1mM β-Mercaptoethanol, 10% glycerol without preservatives.
• Host Species: E. coli
• Purification Method: Ni-NTA agarose
• Quantity: 200 μL
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Content And Storage: Stable for one year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Form: Purified

For Research Use Only