Invitrogen™ Streptavidin, Alexa Fluor™ 430 conjugate

Catalog No.	Quantity
50-112-1176	1 mg

Catalog No. 50-112-1176 Supplier Invitrogen™ Supplier No. S11237

Description

Includes

Three DNA samples: one each from the victim, suspect and crime scene; voucher redeemable by mail for gel cassettes

Invitrogen™'s Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ∼434/539 nm).

Invitrogen™'s Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ∼434/539 nm). Streptavidin, a nonglycosylated biotin-binding protein with a near-neutral isoelectric point, reportedly exhibits less nonspecific binding than avidin.

Specifications

• Conjugate: Alexa Fluor 430
• Detection Method: Fluorescence
• For Use With (Equipment): Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
• Quantity: 1 mg
• Content And Storage: Contains 1 vial of streptavidin Alexa Fluor™ 430 conjugate.
  
  Store in freezer (-5 to -30°C) and protect from light.
• Excitation/Emission: 434/539
• Shipping Condition: Room Temperature
• Form: Solid
• Product Line: Alexa Fluor
• Product Type: Streptavidin Conjugate (Fluorescent)
• Label or Dye: Alexa Fluor Dyes

Frequently Asked Questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

For Research Use Only. Not for use in diagnostic procedures.