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LIFE TECHNOLOGIES E-GEL POWER SNAP SYS EX

Catalog No. g8342st
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Catalog No. G8342ST Supplier LIFE TECHNOLOGIES Supplier No. G8342ST

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Frequently Asked Questions (FAQs)

I am not seeing any current when I try to run my E-Gel EX agarose gels. Why is this?

Here are some common reasons why your gel is not running properly:

- Copper contacts in the base are damaged due to improper use. Make sure the copper contacts in the base are intact.
- An expired or defective gel cassette was used.
- The E-Gel EX cassette was not inserted properly into a base.
- An incorrect adaptor was used.

What is the special feature of E-Gel EX agarose gels?

E-Gel EX agarose gels separate DNA faster, offer enhanced sensitivity, and provide added flexibility. The stain within the EX gels is proprietary (not based on SYBR technology), though it has the same spectral properties as SYBR stains. The E-Gel EX gels have a sensitivity that is 5X greater than gels using ethidium bromide.
I have an E-Gel Simple Runner Electrophoresis Device in my lab, are the new 11-well and 22-well E-Gel agarose gels compatible with it?

Due to the differences in dimensions, the new E-Gel 11-well and 22-well agarose gel formats are not compatible with the E-Gel Simple Runner Electrophoresis Device.

Which DNA ladders do you recommend using with E-Gel EX agarose gels and E-Gel agarose gels with SYBR Safe stain?

To select the DNA ladder that yields the best resolution for your specific E-Gel, please refer to the table on page 45 of the E-Gel Power Snap Electrophoresis System user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017050_egel_powersnapsystem_UG.pdf).
Can I use double comb E-Gel EX agarose gels or E-Gel agarose gels with SYBR Safe stain for RNA analysis?

E-Gel EX Double Comb agarose gels and E-Gel Double Comb agarose gels with SYBR Safe stain have not been tested for use in RNA applications. We recommend using E-Gel EX Single Comb agarose gels with 11 wells for RNA analysis.

Can I dispose of E-Gel EX agarose gels the same way as E-Gel agarose gels with SYBR Safe DNA Gel Stain?

No. Standard safety and hazardous waste disposal procedures should be followed when handling E-Gel EX agarose gels.
What is the difference between E-Gel agarose gels with SYBR Safe DNA Gel Stain and E-Gel EX agarose gels?

E-Gel EX agarose gels separate DNA faster, offer enhanced sensitivity, and provide added flexibility. The stain within the E-Gel EX agarose gels is SYBR Gold II which has similar spectral properties but increased sensitivity compared to SYBR Safe DNA Gel Stain. E-Gel EX agarose gels are especially suited for applications where high sensitivity is critical.
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