Gel Electrophoresis Equipment
Gel Electrophoresis Equipment
Tank assemblies and components for acrylamide, agarose, and IEF electrophoresis of proteins, nucleic acids, and peptides; includes assembly, operation, gel casting, drying, maintenance, and imaging components, replacement parts, and accessories.
Gel electrophoresis is the preferred technique for separating the components of samples that contain nucleic acid (DNA and RNA) and protein macromolecules.
The procedure is performed by placing the sample(s) at one end of an electrophoresis gel in small wells or indentations. An electrical source is attached and runs for a specified timed. A buffer provides ions to help carry the current and maintain pH.
The molecules move based on electrical charge. Proteins are pre-treated with the detergent sodium dodecyl sulfate (SDS) to denature and coat it with a negative charge. Molecules travel at a speed inversely related to size or length, and the degree of separation (distance between the bands) depends on the gel concentration.
Separations usually include electrophoresis(also called DNA ladders or protein ladders) that contain molecules of known sizes and are used for comparison. A variety of buffers and other electrophoresis reagents are also part of the process.
Electrophoresis gels are typically agarose or polyacrylamide; each is suited to different molecules. Polyacrylamide gel electrophoresis (PAGE) is usually used for proteins and smaller DNA fragments. Polyacrylamide gels form by chemical reaction and have uniform pore sizes. They can be cast by the user or purchased as ready-to-use or pre-cast gels.
Agarose gels contain natural polysaccharide polymers from seaweed and have irregular pore sizes. They work well for separating proteins larger than 200 kDa or DNA fragments from 50 to 20,000 bp. Pre-cast agarose gels are available, as are powdered agarose gels for on-site casting.
Polyacrylamide gels are typically run vertically, while agarose gels are run in a horizontal position. Vertical and horizontal electrophoresis equipment is widely available, along with a variety of casting chambers and other electrophoresis system accessories.
Thermo Scientific™ Owl™ D3-14 Wide Gel Electrophoresis System
Screen PCR products, plasmid preps, restriction mapping and cloning with the Thermo Scientific™ Owl™ D3-14 Wide Gel Electrophoresis System.
Fisherbrand™ Horizontal Electrophoresis Systems
Choose from four systems
Fisherbrand™ Real Time Electrophoresis System, with Power Supply
Run DNA gels, view separations as they occur, and capture gel images using a smart phone
Thermo Scientific™ Owl™ EasyCast™ B2 Mini Gel Electrophoresis Systems
Achieve superior flexibility in a small footprint with our B2 mini gel electrophoresis systems.
Axygen™ Horizontal Gel Box, 10 cm
The Axygen HGB-10 is a compact 10cm wide gel box capable of running up to 100 samples depending on configuration. System is ideal for quick sample-checks, particularly following restriction digestion during cloning. Comes with two different size UV transparent gel trays providing flexibility and value.
Promotions are available
Fisherbrand™ Electrophoresis Power Supplies
Easy-to-use power supplies designed for safety
Axygen™ Horizontal Gel Box, 15 cm
The Axygen HGB-15 is large sample capacity system ideal for screening PCR samples or routine DNA fragment analysis and comes standard with two different size UV transparent gel trays providing flexibility and value.
Promotions are available
Axygen™ Horizontal Gel Box, 20 cm
The Axygen HGB-20 allows for high resolution separations over extended runs. System is ideal for separating a high number of samples from PCR or cloning. Comes with two different size UV transparent gel trays providing flexibility and value.
Promotions are available
Fisherbrand™ Mini Gel System Casting Set
For use with Fisherbrand Horizontal Mini Gel Electrophoresis System, this cast set includes a casting stand, 10.5x6cm gel trays and dual sided combs. The trays feature a dark contrast strip to help visualize wells during sample loading and fluorescent lines to track the progress of a run.
Fisherbrand™ Vertical Electrophoresis Systems
Choice of 10 × 10cm, 16 × 14cm, or 20 × 20cm gel size
Fisherbrand™ Real Time Electrophoresis Tank System
Run DNA gels, view separations as they occur, and capture gel images using a smart phone
Fisherbrand™ Horizontal Mini Gel Electrophoresis System
Designed for quick separation of DNA or nucleic acids in agarose mini-gels, the Fisherbrand Mini Horizontal Gel System is a complete, compact mini gel electrophoresis system for easy nucleic acid separation. The horizontal system comes with an attached power supply, two casting stands, six gel trays and four combs — everything necessary for DNA or nucleic acid, horizontal electrophoresis.
Thermo Scientific™ Owl™ EasyCast™ B1 Mini Gel Electrophoresis Systems
Cast and run a gel in the same chamber with no tape or additional parts required with the leakproof casting of mini gel electrophoresis systems.
Thermo Scientific™ Nalgene™ Staining Box
Stain, fix and handle fragile electrophoresis gels and membranes with less chance of damage than conventional methods with these staining boxes.
Thermo Scientific™ Owl™ D2 Wide-Gel Electrophoresis System
Run 10 to 80 samples on one gel with this wide-gel electrophoresis system.