Invitrogen™ E4BP4 (NFIL3) Monoclonal Antibody (S2M-E19), Alexa Fluor™ 647, eBioscience™

Catalog No.	Quantity
51-592-782	100 μg

Catalog No. 51-592-782 Supplier Invitrogen™ Supplier No. 51592782

Description

Rat Monoclonal Antibody

This S2M-E19 monoclonal antibody recognizes mouse E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3. E4BP4 is involved in the transcriptional control of the mammalian circadian clock, motor neuron growth and survival, regulation of von Willbrand factor expression, and osteoblast function. In addition, it is also required for the development of NK and NKT cells as well as CD8a+ dendritic cells. Although relatively high levels of mRNA are reported in resting NK and NKT cells, studies at eBioscience have observed expression of E4BP4 protein in lymphocytes only after activation. Applications Reported: This S2M-E19 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This S2M-E19 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins' located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can ra...

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).

Specifications

• Antigen: E4BP4 (NFIL3)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: S2M-E19
• Concentration: 0.2 mg/mL
• Conjugate: Alexa Fluor 647
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: NFIL3
• Gene Accession No.: O08750
• Gene Alias: AV225605; E4 promoter binding-protein 4; E4 promoter-binding protein 4; E4BP4; Embryo implantation-related NFIL3/E4BP4-like transcription factor; IL3BP1; Interleukin-3 promoter transcriptional activator; interleukin-3-binding protein 1; NF IL3A; NFIL3; NFIL3A; NF-IL3A; nuclear factor interleukin 3 regulated protein; nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; nuclear factor, interleukin 3, regulated; OTTHUMP00000021633; transcriptional activator NF-IL3A
• Gene Symbols: NFIL3
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18030
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ