Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Catalog No.	Quantity	Excitation/Emission	Conjugate
50-930-1	300 Tests	488, 535/520, 617	FITC, Propidium Iodide
50-927-5	200 Tests	488, 535/520, 617	FITC, Propidium Iodide
50-927-6	50 Tests	405, 546/450, 647	eFluor 450, 7-AAD
50-927-7	200 Tests	405, 546/450, 647	eFluor 450, 7-AAD
50-927-9	200 Tests	650, 535/660, 617	APC, Propidium Iodide
50-928-0	50 Tests	-	PCP-eFluor 710
50-928-1	200 Test	-	PCP-eFluor 710
50-929-7	100 Tests	488, 535/520, 617	FITC, Propidium Iodide
50-929-9	20 Tests	488, 535/520, 617	FITC, Propidium Iodide
50-246-343	50 Tests	-	PE, 7-AAD
50-246-344	200 Tests	-	PE, 7-AAD
50-246-345	50 Tests	-	PE/Cy7
50-246-346	200 Tests	-	PE/Cy7
50-929-4	100 Tests	NA, 535/NA, 617	Biotin, Propidium Iodide
50-929-5	20 Tests	NA, 535/NA, 617	Biotin, Propidium Iodide
50-929-6	300 Tests	NA, 535/NA, 617	Biotin, Propidium Iodide
50-112-8958	50 Tests	488, 535/520, 617	FITC, Propidium Iodide
50-112-9048	50 Tests	650, 535/660, 617	APC, Propidium Iodide

Catalog No. 50-930-1 Supplier Invitrogen™ Supplier No. BMS500FI300

Description

Includes

• Annexin V-FITC protein
• Binding Buffer (4x)
• Propidium Iodide (20 ug/mL)

Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.

Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.

Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

Specifications

• Description: eBioscience Annexin V-FITC Apoptosis Detection Kit
• Quantity: 300 Tests
• Kit Contents: Contains:
  • Binding Buffer (4X), 1 bottle (50 mL)
  • rh Annexin V-FITC, 1 vial (1.5 mL)
  • Propidium Iodide (20 μg/mL), 2 vials (1.8 mL)
• Product Type: Apoptosis Detection Kit
• Content And Storage: Handling Conditions:
  Store at 2-8°C. Avoid temperature fluctuations.
• Excitation/Emission: 488, 535/520, 617
• Shipping Condition: Wet Ice
• Conjugate: FITC, Propidium Iodide
• For Use With (Equipment): Flow Cytometer