Investigating Synthetic Biology

Cloning experiment for the classroom.

Supplier:  Edvotek 331

Catalog No. S66821

Includes: PCR EdvoBeads™, primer mix concentrate, template DNA concentrate, universal DNA buffer, ultrapure water, DNA standard marker, 10X gel loading solution, purification and wash buffers, spin columns and reservoirs, Dryzyme™ mix, Dryzyme™ reconstitution buffer, enzyme reaction buffer, vector cut with EcoRI and HindIII, T4 DNA ligase, ReadyPour LB agar, LB medium, BactoBeads™ (Host), Calcium Chloride, competent cell solution, control DNA, recovery broth, Kanamycin, IPTG, Salacylic acid, wintergreen scent, 50X electrophoresis buffer, UltraSpec-Agarose™, SYBR™ Safe Stain, microcentrifuge and PCR tubes, 10 ml pipet, 50 ml conical tubes, sterile loops

Description

Teach your students about synthetic biology with this exciting and exclusive lab! Students use PCR to amplify the coding sequence of the BSMT1 enzyme. This interesting enzyme is responsible for the formation of methyl salicylate, a chemical with a strong 'wintergreen' odor. The PCR product is purified, restriction digested, and inserted into a plasmid vector. The resulting recombinant DNA is then used to transform E. coli BactoBeads™. Finally, students design an experiment to express the enzyme from their transformants and perform a smell test to confirm that the bacterial factories are working!

Specifications

• Content And Storage: Instructions, biologicals, Buffers and reagents for PCR, ligation and transformation, ReadyPour™ luria broth agar, DNA size ladder, wax beads, Agarose, electrophoresis Buffer, Gel stain. Store at -20°C
• Form: Gel
• PCR Method: Standard PCR
• Label or Dye: SYBR Green, FlashBlue Stain
• Quantity: 1 Kit

• Detection Method: Gel Electrophoresis
• GC-Rich PCR Performance: Medium
• Reaction Speed: 30 to 90 min.
• Product Type: Classroom Experiment
• For Use With (Application): Standard PCR