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Investigating Synthetic Biology
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Catalog No. S66821 Shop All EDVOTEK Products
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PCR amplified gene cloning kit
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S66821 PCR amplified gene cloning kit
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Catalog No. S66821 Supplier Edvotek Supplier No. 331
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Description

Includes

PCR EdvoBeads™, primer mix concentrate, template DNA concentrate, universal DNA buffer, ultrapure water, DNA standard marker, 10X gel loading solution, purification and wash buffers, spin columns and reservoirs, Dryzyme™ mix, Dryzyme™ reconstitution buffer, enzyme reaction buffer, vector cut with EcoRI and HindIII, T4 DNA ligase, ReadyPour LB agar, LB medium, BactoBeads™ (Host), Calcium Chloride, competent cell solution, control DNA, recovery broth, Kanamycin, IPTG, Salacylic acid, wintergreen scent, 50X electrophoresis buffer, UltraSpec-Agarose™, SYBR™ Safe Stain, microcentrifuge and PCR tubes, 10 ml pipet, 50 ml conical tubes, sterile loops

Requires

  • Horizontal gel electrophoresis apparatus
  • Thermal cycler
  • Water baths
  • Incubation oven
  • Hotplate
  • Micropipets
  • Balance
  • UV transilluminator
  • Microtest tubes
  • 250mL flasks
  • Ice
  • All necessary safety equipment
  • Distilled or deionized water

Cloning experiment for the classroom.

Teach your students about synthetic biology with this exciting and exclusive lab! Students use PCR to amplify the coding sequence of the BSMT1 enzyme. This interesting enzyme is responsible for the formation of methyl salicylate, a chemical with a strong 'wintergreen' odor. The PCR product is purified, restriction digested, and inserted into a plasmid vector. The resulting recombinant DNA is then used to transform E. coli BactoBeads™. Finally, students design an experiment to express the enzyme from their transformants and perform a smell test to confirm that the bacterial factories are working!

Specifications

Content And Storage Instructions, biologicals, Buffers and reagents for PCR, ligation and transformation, ReadyPour™ luria broth agar, DNA size ladder, wax beads, Agarose, electrophoresis Buffer, Gel stain. Store at -20°C
Detection Method Gel Electrophoresis
Form Gel
GC-Rich PCR Performance Medium
PCR Method Standard PCR
Reaction Speed 30 to 90 min.
Label or Dye SYBR Green, FlashBlue Stain
Product Type Classroom Experiment
Quantity 1 Kit
For Use With (Application) Standard PCR
Product Title
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