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Edvotek™ Drosophila Genotyping Using PCR
SDPSCI_ED

Catalog No. S66827 Shop All EDVOTEK Products
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Drosophla Genotyping Pcr
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S66827 Drosophla Genotyping Pcr
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Catalog No. S66827 Supplier Edvotek Supplier No. 337
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Description

Includes

  • Flies
  • Primers
  • Buffers
  • Chemicals
  • Instructions and background information
  • Study questions

Requires

  • Horizontal gel electrophoresis apparatus
  • Thermal cycler
  • Hotplate
  • Micropipetter
  • Balance
  • Water bath
  • Microcentrifuge
  • 500mL flasks
  • UV transilluminator
  • Pipet pump or bulb
  • Isopropanol
  • All necessary safety equipment
  • Distilled or deionized water

Advanced-level experiment uses agarose gel electrophoresis to explore DNA polymorphisms.

Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.

Order Info

Class size: 10 groups (20 PCR reactions).

Specifications

Content And Storage Instructions, PCR EdvoBeads™, primers, DNA extraction Buffer, 200bp ladder, proteinase K, drosophila (wild type and white eye), Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide and FlashBlue™ Stain. Store at -20°C
Detection Method Gel Electrophoresis
Form Lyophilized
GC-Rich PCR Performance Medium
PCR Method Standard PCR
Reaction Speed 30 to 90 min.
Label or Dye SYBR Green, FlashBlue Stain
Product Type Classroom Experiment
Quantity 1 Kit
For Use With (Application) Genotyping
No. of Reactions 20
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