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Edvotek™ Drosophila Genotyping Using PCR
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Drosophla Genotyping Pcr
Description
Includes
- Flies
- Primers
- Buffers
- Chemicals
- Instructions and background information
- Study questions
Requires
- Horizontal gel electrophoresis apparatus
- Thermal cycler
- Hotplate
- Micropipetter
- Balance
- Water bath
- Microcentrifuge
- 500mL flasks
- UV transilluminator
- Pipet pump or bulb
- Isopropanol
- All necessary safety equipment
- Distilled or deionized water
Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.
Order Info
Class size: 10 groups (20 PCR reactions).
Specifications
Specifications
| Content And Storage | Instructions, PCR EdvoBeads™, primers, DNA extraction Buffer, 200bp ladder, proteinase K, drosophila (wild type and white eye), Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide and FlashBlue™ Stain. Store at -20°C |
| Detection Method | Gel Electrophoresis |
| Form | Lyophilized |
| GC-Rich PCR Performance | Medium |
| PCR Method | Standard PCR |
| Reaction Speed | 30 to 90 min. |
| Label or Dye | SYBR Green, FlashBlue Stain |
| Product Type | Classroom Experiment |
| Quantity | 1 Kit |
| For Use With (Application) | Genotyping |
| Show More |
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