Edvotek™ Mitochondrial DNA Analysis Using PCR

Catalog No. S66822

Supplier: Edvotek 332

Description

Includes

Instructions, PCR EdvoBeads™, Mitochondrial Primer Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & Wax Beads

Requires

• Thermal cycler
• Horizontal gel electrophoresis apparatus
• Microcentrifuge
• Hotplate
• Micropipets
• Balance
• Water bath
• UV transilluminator
• 500mL flasks
• All necessary safety equipment
• Distilled or deionized water

Advanced-level experiment uses the Polymerase Chain Reaction (PCR) to amplify a specific region of the mitochondrial chromosome and perform gel electrophoresis.

• Energy within the cell is produced by the organelles called mitochondria, which have their own DNA (mtDNA)
• Mitochondrial diseases have been mapped to the mtDNA chromosome
• Forensic identification also commonly examines mtDNA

Order Info

Contains materials for 25 individual reactions and six gels.

Specifications

• Content And Storage: Instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, chelating agent, Agarose, DNA ladder, practice Gel loading solution, Gel loading dye, Buffer, InstaStain™ Ethidium Bromide and FlashBlue™ Stain. Store at -20°C
• Detection Method: Gel Electrophoresis
• Form: Lyophilized
• GC-Rich PCR Performance: Medium
• PCR Method: Standard PCR
• Reaction Speed: 30 to 90 min.
• Label or Dye: SYBR Green, FlashBlue Stain
• Product Type: Classroom Experiment
• Quantity: 1 Kit
• For Use With (Application): Standard PCR
• No. of Reactions: 25