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Edvotek™ Mitochondrial DNA Analysis Using PCR
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Catalog No. S66822 Shop All EDVOTEK Products
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Mitochondrial DNA Analysis Using PCR
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S66822 Mitochondrial DNA Analysis Using PCR
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Catalog No. S66822 Supplier Edvotek Supplier No. 332
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Description

Includes

Instructions, PCR EdvoBeads™, Mitochondrial Primer Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & Wax Beads

Requires

  • Thermal cycler
  • Horizontal gel electrophoresis apparatus
  • Microcentrifuge
  • Hotplate
  • Micropipets
  • Balance
  • Water bath
  • UV transilluminator
  • 500mL flasks
  • All necessary safety equipment
  • Distilled or deionized water

Advanced-level experiment uses the Polymerase Chain Reaction (PCR) to amplify a specific region of the mitochondrial chromosome and perform gel electrophoresis.

  • Energy within the cell is produced by the organelles called mitochondria, which have their own DNA (mtDNA)
  • Mitochondrial diseases have been mapped to the mtDNA chromosome
  • Forensic identification also commonly examines mtDNA

Order Info

Contains materials for 25 individual reactions and six gels.

Specifications

Content And Storage Instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, chelating agent, Agarose, DNA ladder, practice Gel loading solution, Gel loading dye, Buffer, InstaStain™ Ethidium Bromide and FlashBlue™ Stain. Store at -20°C
Detection Method Gel Electrophoresis
Form Lyophilized
GC-Rich PCR Performance Medium
PCR Method Standard PCR
Reaction Speed 30 to 90 min.
Label or Dye SYBR Green, FlashBlue Stain
Product Type Classroom Experiment
Quantity 1 Kit
For Use With (Application) Standard PCR
No. of Reactions 25
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