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Edvotek™ PCR-Based DNA Typing
DNA typing adapted from FBI technology.
Supplier: Edvotek 334
Includes: Instructions, PCR EdvoBeads™, D1S80 Primer Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, 10x Gel Loading Solution, InstaStain™ Ethidium Bromide, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & Wax Beads
Requires :
- Thermal cycler
- Horizontal gel electrophoresis apparatus
- Microcentrifuge
- Automatic micropipets
- UV transilluminator
- 500mL flasks
- Glassware
- All necessary safety equipment
- Ice
- Ice bucket
- Distilled or deionized water
Description
Safe experiment for classroom use to demonstrate DNA fingerprinting.
Alu-Human DNA Typing (S66823)
- Examine students' DNA for an Alu element on Chromosome 16
- Investigate DNA patterns
- Compare Alu genotypes with those of their classmates
VNTR Human DNA Typing (S66824)
- Use PCR to fingerprint students' DNA at the D1S80 locus on Chromosome 1
- Provide an example of the variable number of tandem repeat (VNTR) polymorphism
Specifications
| Instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, chelating agent, Agarose, DNA ladder, practice Gel loading solution, Gel loading dye, Buffer, FlashBlue™ stain. Store at -20°C | |
| Lyophilized | |
| Standard PCR | |
| SYBR Green, FlashBlue Stain | |
| 1 Kit | |
| 25 |
| Gel Electrophoresis | |
| Medium | |
| 60 min. | |
| Classroom Experiment | |
| Standard PCR |
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