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Edvotek™ PCR-Based DNA Typing
SDPSCI_ED

Catalog No. S66824 Shop All EDVOTEK Products
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Content And Storage:
Instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, chelating agent, Agarose, DNA ladder, practice Gel loading solution, Gel loading dye, Buffer, FlashBlue™ stain. Store at -20°C
Instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, chelating agent, Agarose, DNA ladder, practice Gel loading solution, Gel loading dye, Buffer, InstaStain™ Ethidium Bromide and FlashBlue™ Stain. Store at -20°C

Description

Includes

Instructions, PCR EdvoBeads™, D1S80 Primer Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, 10x Gel Loading Solution, InstaStain™ Ethidium Bromide, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & Wax Beads

Requires

  • Thermal cycler
  • Horizontal gel electrophoresis apparatus
  • Microcentrifuge
  • Automatic micropipets
  • UV transilluminator
  • 500mL flasks
  • Glassware
  • All necessary safety equipment
  • Ice
  • Ice bucket
  • Distilled or deionized water

DNA typing adapted from FBI technology.

Safe experiment for classroom use to demonstrate DNA fingerprinting.

Alu-Human DNA Typing (S66823)

  • Examine students' DNA for an Alu element on Chromosome 16
  • Investigate DNA patterns
  • Compare Alu genotypes with those of their classmates

VNTR Human DNA Typing (S66824)

  • Use PCR to fingerprint students' DNA at the D1S80 locus on Chromosome 1
  • Provide an example of the variable number of tandem repeat (VNTR) polymorphism

Specifications

Content And Storage Instructions, proteinase K, PCR EdvoBeads™, control DNA and primers, microtubes, chelating agent, Agarose, DNA ladder, practice Gel loading solution, Gel loading dye, Buffer, FlashBlue™ stain. Store at -20°C
Detection Method Gel Electrophoresis
Form Lyophilized
GC-Rich PCR Performance Medium
PCR Method Standard PCR
Reaction Speed 60 min.
Label or Dye SYBR Green, FlashBlue Stain
Product Type Classroom Experiment
Quantity 1 Kit
For Use With (Application) Standard PCR
No. of Reactions 25
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