Invitrogen™ EEA1 Monoclonal Antibody (F.43.1)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA514794

Catalog No. PIMA514794

Description

It is not recommended to aliquot this antibody.

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

Specifications

• Antigen: EEA1
• Classification: Monoclonal
• Concentration: 44 μg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: Q15075, Q8BL66
• Gene Symbols: EEA1
• Immunogen: Synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: F.43.1
• Conjugate: Unconjugated
• Gene: EEA1
• Gene Alias: A430109M19Rik; B230358H09Rik; Early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; EEA1; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain-containing protein 2
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 216238, 314764, 8411
• Content And Storage: -20°C
• Form: Liquid