Invitrogen™ Human EG-VEGF/PROK1 ELISA Kit

Catalog No.	Quantity
EHPROK1X5	5 x 96 Tests

Catalog No. EHPROK1X5 Supplier Invitrogen™ Supplier No. EHPROK1X5

Description

ELISA

The Human EG-VEGF/PROK1 ELISA quantitates Hu EG-VEGF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EG-VEGF. Principle of the method The Human EG-VEGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Endocrine gland-derived vascular endothelial growth factor induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands. Its expression is often complementary to the expression of VEGF, suggesting that these molecules function in a coordinated manner.

Specifications

• Accession Number: black mamba toxin-related protein; EGVEGF; EG-VEGF; Endocrine-gland-derived vascular endothelial growth factor; mambakine; Pk1; PRK1; PROK1; prokineticin 1; prokineticin-1; UNQ600/PRO1186
• Assay Range: 13.72 to 10,000 pg/mL
• Assay Sensitivity: 10 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 84432
• Gene Symbol: PROK1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: PROK1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins