Novus Biologicals™ EGFR Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human EGFR.

Specifications

• Assay Range: 46.88 pg/mL
• Assay Sensitivity: 46.88-3000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human EGFR.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• Gene ID (Entrez): 1956
• Gene Symbol: EGFR
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 45 ng of recombinant Human EGFR.
  Capture Antibody: 1 mg/mL of rabbit anti-Human EGFR monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human EGFR monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Cellular Signaling, Growth and Development, Hypoxia, Phospho Specific, Signal Transduction, Tumor Suppressors, Tyrosine Kinases
• Storage Requirements: Storage is content dependent.
• Target: EGFR
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.