EGR2 Monoclonal Antibody (erongr2), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 17669182

Catalog No. 50-112-3179

Description

Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predomitly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis. Applications Reported: This erongr2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This erongr2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523). This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

EGR2 Monoclonal antibody specifically detects EGR2 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: EGR2
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P08152
• Gene Symbols: Egr2
• Immunogen: E. coli-derived protein, aa 1-319
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: erongr2
• Conjugate: APC
• Gene: Egr2
• Gene Alias: AT591; CMT1D; CMT4E; DKFZp686J1957; E3 SUMO-protein ligase EGR2; E3 SUMO-protein transferase ERG2; early growth response 2; early growth response protein 2; Egr2; Egr-2; FLJ1454; FLJ14547; Krox20; Krox-20; Krox-20 homolog; KROX-20, Drosophila, homolog (early growth response-2); NGF1-B; OTTHUMP00000062133; Zfp-25; Zfp-6; Zinc finger protein Krox-20
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13654
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

For Research Use Only.