Invitrogen™ eIF3e Monoclonal Antibody (10F5H6)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549155

Catalog No. PIMA549155

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse and rat EIF3e. Positive Control - WB: human Raji whole cell, human K562 whole cell, rat thymus tissue, mouse thymus tissue, mouse ANA-1 whole cell, mouse SP2/0 whole cell. Flow: U20S cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Specifications

• Antigen: eIF3e
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P60228, P60229, Q641X8
• Gene Symbols: EIF3E
• Immunogen: E.coli-derived human EIF3e recombinant protein (Position: A160-Q241).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Western Blot
• Clone: 10F5H6
• Conjugate: Unconjugated
• Gene: EIF3E
• Gene Alias: 48kDa; eIF-3 p48; eIF3e; eIF3-p46; EIF3-P48; EIF3S6; eukaryotic translation initiation factor 3 subunit 6; Eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3 subunit E isoform 2 transcript; eukaryotic translation initiation factor 3 subunit E-like protein; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; eukaryotic translation initiation factor 3, subunit E; eukaryotic translation initiation factor subunit E-like protein; Int6; mammary tumor integration site 6; mammary tumor-associated protein INT6; mammary tumor-associated protein INT-6; MMTV integration site 6; murine mammary tumor integration site 6 (oncogene homolog); NEWGENE_1311521; Viral integration site protein INT-6; viral integration site protein INT-6 homolog
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 103692906, 16341, 3646
• Content And Storage: -20°C
• Form: Lyophilized