Invitrogen™ γ-Glutamyl Transferase (GGT/γ-GT) Colorimetric Activity Kit

Catalog No.	Quantity
EEA029	96 tests

Catalog No. EEA029 Supplier Invitrogen™ Supplier No. EEA029

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The γ-Glutamyl Transferase (GGT/γ-GT) Colorimetric Activity Kit is a highly reliable and sensitive assay designed for the quantitative measurement of GGT in serum, plasma, and animal tissue samples. GGT is widely present in various organs of the human body, including the liver, kidney, and pancreas. GGT catalyzes the degradation of glutathione (GSH) and is involved in the regulation of glutathione levels in tissues, as well as the absorption and excretion of amino acids. Changes in GGT activity have been associated with various diseases, particularly those affecting the hepatobiliary system.

The assay utilizes the catalytic property of GGT to transfer a γ-glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine, resulting in the production of p-nitroaniline. The p-nitroaniline has a characteristic absorption peak at 405 nm, allowing for the measurement of GGT activity.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.88–399.4 U/L
• Assay Sensitivity: 0.88 U/L
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Plasma, Serum, Tissue Homogenates
• For Use With (Equipment): Microplate Reader
• Incubation Time: 6 min.
• Interassay CV: 6.2
• Intraassay CV: 4.2
• Quantity: 96 tests
• Sample Volume: 25 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: γ-Glutamyl Transferase (GGT/γ-GT)
• Total Assay Time: 20 min.