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Invitrogen™ High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit

Catalog No. EEA012
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EEA012 96 tests
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Catalog No. EEA012 Supplier Invitrogen™ Supplier No. EEA012
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Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of HDL-C in serum, plasma, culture supernatant, tissue, and cells. HDL-C is an anti-atherosclerotic lipoprotein that can transport cholesterol from extrahepatic tissues to the liver for metabolism and excretion of bile from the body. Its plasma level is negatively correlated with the risk of cardiovascular disease.

The assay is based on the reaction of HDL-C with enzyme reagents containing cholesterol esterase and cholesterol oxidase to produce hydrogen peroxide. The hydrogen peroxide is catalyzed by an oxidase in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound. The colored substance has a maximum absorption peak at 546 nm. The HDL-C content in the sample can be calculated by measuring the OD value at 546 nm.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 0.065–3.8 mmol/L
Assay Sensitivity 0.065 mmol/L
Content And Storage Store at 2–8°C.
Detection Method Colorimetric
Sample Type Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Whole Cells
For Use With (Equipment) Microplate Reader
Incubation Time 10 min.
Interassay CV 5
Intraassay CV 3
Quantity 96 tests
Sample Volume 2.5 μL
Shipping Condition Dry Ice, 2–8°C
Target Kit Named High-density Lipoprotein Cholesterol (HDL-C)
Total Assay Time 20 min.
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