Invitrogen™ Lactate Dehydrogenase (LDH) Colorimetric Activity Kit

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Lactate Dehydrogenase (LDH) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of LDH in serum, plasma, tissue, and cell culture samples. LDH is an enzyme that catalyzes the conversion of lactate to pyruvate, an essential step in cellular energy metabolism. Elevated LDH levels are associated with tissue damage, inflammation, and certain diseases, making it a useful biomarker in clinical and research settings.

In this assay, LDH catalyzes lactic acid to produce pyruvate. Pyruvate reacts with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which is red-brown in alkaline solution. The color intensity is proportional to pyruvate concentration, where the activity of LDH can be calculated by measuring OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 6–1000 U/L
• Assay Sensitivity: 6 U/L
• Content And Storage: Store at 2–8°C.
  
  
• Detection Method: Colorimetric
• Sample Type: Hydrothorax, Plasma, Serum, Tissue Homogenates, Whole Cells
• For Use With (Equipment): Microplate Reader
• Incubation Time: 35 min.
• Interassay CV: 2.4
• Intraassay CV: 1.8
• Quantity: 96 tests
• Sample Volume: 40 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Lactate Dehydrogenase (LDH)
• Total Assay Time: 45 min.