Invitrogen™ Total Cholesterol (TC) Colorimetric Assay Kit (single reagent, COD-PAP method)

Catalog No.	Quantity
EEA026	96 tests

Catalog No. EEA026 Supplier Invitrogen™ Supplier No. EEA026

Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Total Cholesterol (TC) Colorimetric Assay Kit (single reagent, COD-PAP method) is a highly reliable and sensitive assay designed for the quantitative measurement of total cholesterol in serum, plasma, and animal tissue. Cholesterol is a type of sterol and lipid found in cell membranes. It is an essential component of cell structure and function, but high levels of TC in the blood have been linked to an increased risk of cardiovascular disease. The measurement of TC levels (which includes free cholesterol and cholesterol esters) is an important diagnostic tool in clinical and research settings.

The assay utilizes the COD-PAP method, which involves the hydrolysis of cholesterol esters in the sample to produce free cholesterol and free fatty acids. Free cholesterol is then oxidized by cholesterol oxidase to produce 4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyzes peroxidase to form red quinone compounds. The color depth of the generated quinone is directly proportional to the cholesterol content, which can be measured spectrophotometrically at 510 nm.

Key features

• Simple and fast protocol – user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits – across key analytes and targets within the most important metabolic pathways
• Consistent – strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.29 to 25.85 mmol/L
• Assay Sensitivity: 0.29 mmol/L
• Content And Storage: Store at 2°C to 8°C.
• Detection Method: Colorimetric
• Sample Type: Plasma, Serum, Tissue Homogenates
• For Use With (Equipment): Microplate Reader
• Incubation Time: 10 min.
• Interassay CV: 8.3
• Intraassay CV: 3.1
• Quantity: 96 tests
• Sample Volume: 2.5 μL
• Shipping Condition: Dry Ice, 2–8°C
• Target Kit Named: Total Cholesterol (TC)
• Total Assay Time: 20 min.

Frequently Asked Questions (FAQs)

Can I use the Total Cholesterol (TC) Colorimetric Assay Kit (single reagent, COD-PAP method) (Cat. No. EEA026) with cell samples?

We do not recommend using the Total Cholesterol (TC) Colorimetric Assay Kit with cell samples because most untreated cell samples have a low TC that does not meet the sensitivity of the kit.