Thermo Scientific™ E. coli DNA Ligase

Description

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.

Features

• Activity in wide temperature range (4°C to 37°C)
• High specificity to cohesive DNA ends

Applications

• Nick ligation in double-stranded DNA
• cDNA synthesis
• Cloning

Specifications

• Concentration: 8 U/μL
• For Use With (Application): Nick ligation in double-stranded DNA, cDNA synthesis, Cloning, DNA ligation
• Content And Storage: 8 U/μL E.Coli DNA Ligase, 50 μL
  
  Store at -20°C.
• Enzyme: DNA Ligase
• Sensitive to Heat Inactivation: Yes
• Quantity: 400 U
• Product Type: NAD-dependent DNA Ligase
• Research Category: Traditional Cloning
• Inactivation Temperature: 65°C
• Form: Liquid

For Research Use Only. Not for use in diagnostic procedures.