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Thermo Scientific™ Thermus thermophilus DNA Ligase

Catalog No. FEREL07981
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1000 U
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FEREL07981 1000 U
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Catalog No. FEREL07981 Supplier Thermo Scientific™ Supplier No. EL07981
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Description

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. Thermus thermophilus DNA Ligase is not active on RNA, single-stranded DNA, or blunt-ended DNA.

Features

  • Increased specificity—high ligation temperature eliminates non-specific ligation
  • Stabile at high temperatures with an optimum ligation temperature of 45°C to 65°C

Applications

Thermus thermophilus DNA ligase is recommended for applications requiring high ligation specificity, including:

  • Single-stranded nick repair in double-stranded DNA
  • Next-generation sequencing
  • Rolling circle amplification
  • Proximity ligation assay
TRUSTED_SUSTAINABILITY

Specifications

Concentration 50 U/μL
For Use With (Application) Single-stranded nick repair, NGS, RCA, PLA
Content And Storage 10X Thermus thermophilus DNA Ligase Buffer, 40 μL

Store at -20°C.
Enzyme DNA Ligase
Sensitive to Heat Inactivation No
Quantity 1000 U
Product Type NAD-dependent DNA Ligase
Research Category Next-generation Sequencing, DNA Repair
Form Liquid

For Research Use Only. Not for use in diagnostic procedures.

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