Thermo Scientific™ Thermus thermophilus DNA Ligase

Description

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. Thermus thermophilus DNA Ligase is not active on RNA, single-stranded DNA, or blunt-ended DNA.

Features

• Increased specificity—high ligation temperature eliminates non-specific ligation
• Stabile at high temperatures with an optimum ligation temperature of 45°C to 65°C

Applications

Thermus thermophilus DNA ligase is recommended for applications requiring high ligation specificity, including:

• Single-stranded nick repair in double-stranded DNA
• Next-generation sequencing
• Rolling circle amplification
• Proximity ligation assay

Specifications

• Concentration: 50 U/μL
• For Use With (Application): Single-stranded nick repair, NGS, RCA, PLA
• Content And Storage: 10X Thermus thermophilus DNA Ligase Buffer, 40 μL
  
  Store at -20°C.
• Enzyme: DNA Ligase
• Sensitive to Heat Inactivation: No
• Quantity: 1000 U
• Product Type: NAD-dependent DNA Ligase
• Research Category: Next-generation Sequencing, DNA Repair
• Form: Liquid

For Research Use Only. Not for use in diagnostic procedures.