MilliporeSigma™ Cathepsin V, HisTag™, Human, Recombinant Protein, NSO Cells

Catalog No.	Quantity
21-946-710UG	10 μg

Catalog No. 21-946-710UG Supplier MilliporeSigma™ Supplier No. 21946710UG

Description

Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His·Tag™ sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L, these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ∽40kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.

Specifications

• Concentration: Concentration on Label
• Formulation: In 800mM NaCl, 25mM sodium acetate, 0.8M NaCl, pH 5.0.
• Purification Method: SDS-PAGE
• Quantity: 10 μg
• Immunogen: Cathepsin V
• Storage Requirements: To activate rhCathepsin V, pre-incubate it at 20μg/mL in 25mM NaOAc, 100mM NaCl, 5mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (−70°C).
• Regulatory Status: RUO
• Cell Type: NSO Cells
• Conjugate: His-Tag
• Species: Human
• Recombinant: Recombinant
• Form: Frozen
• Purity or Quality Grade: ≥95%