MilliporeSigma™ Complement C3b, Human, Protein

Description

Native, human C3b complement component. Cleavage of the C3-α chain at peptide bond 77 by either of the complement C3 convertase enzymes results in production of C3a (M.W. 9083) and C3b (M.W. 180,000) fragments. The C3b fragment is a glycoprotein composed of the modified C3-α chain (α') (M.W. 105,000) and the intact C3-β chain (M.W. 75,000). Nascent C3b has the transient ability to form a covalent ester bond with a variety of target surfaces. Once bound to target surfaces C3b becomes an essential subunit of both the classical and alternative pathway C5 cleaving enzymes. In addition, surface-bound C3b has opsonic and immune adherence activities which are mediated via binding to CR1 (CD35) complement receptors.

Specifications

• Formulation: In PBS, pH 7.2, filtered through a 0.22μM filter.
• Purification Method: SDS-PAGE
• Quantity: 250 μg
• Immunogen: Complement C3b
• Storage Requirements: Following initial thaw, aliquot and freeze (−70°C).
• Regulatory Status: RUO
• Species: Human
• Recombinant: Native
• Form: Liquid
• Purity or Quality Grade: ≥90%