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MilliporeSigma™ Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant Protein, E. coli

Catalog No. 324725700MI Shop All MilliporeSigma Products
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700 mIU
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32-472-5700MI 700 mIU
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Description

Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F and therefore is more suitable for deglycosylation of native proteins.
TRUSTED_SUSTAINABILITY

Specifications

Formulation In 20mM Tris-HCl, pH 7.5.
Quantity 700 mIU
Source E. coli
Immunogen Endoglycosidase F1
Regulatory Status RUO
Species E. meningosepticum
Recombinant Recombinant
Form Liquid
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