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MilliporeSigma™ Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant Protein, E. coli

Catalog No. 324727200MI Shop All MilliporeSigma Products
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32-472-7200MI 200 mIU
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Catalog No. 32-472-7200MI Supplier MilliporeSigma™ Supplier No. 324727200MIU
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Recombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F and therefore is more suitable for deglycosylation of native proteins.
TRUSTED_SUSTAINABILITY

Specifications

Formulation In 20mM Tris-HCl, pH 7.5.
Quantity 200 mIU
Source E. coli
Immunogen Endoglycosidase F3
Regulatory Status RUO
Species E. meningosepticum
Recombinant Recombinant
Form Liquid
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