MilliporeSigma™ Leptin, Human, Recombinant Protein, E. coli

Catalog No.	Quantity
42-970-01MG	1 mg

Catalog No. 42-970-01MG Supplier MilliporeSigma™ Supplier No. 4297001MG

Description

Recombinant, human leptin expressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations of the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis. Note: Following complete dissolution in 15mM HCl, add 7.5mM sterile NaOH and bring the pH to approximately 5.2.

Specifications

• Formulation: Lyophilized from a sterile filtered solution in PBS.
• Purification Method: SDS-PAGE
• Quantity: 1 mg
• Source: E. coli
• Immunogen: Leptin
• Storage Requirements: To reconstitute lyophilized leptin, add 150mM sterile HCl (0.5mL/1mg vial or 2.5mL/5mg vial) to the vial. After the protein is completely dissolved, add 7.5mM sterile NaOH (0.3mL/1mg vial or 1.5mL/5mg vial) to bring the pH to ∽5.2. Lyophilized samples are stable for at least six months at −70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at −70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
• Regulatory Status: RUO
• Species: Human
• Recombinant: Recombinant
• Form: Lyophilized
• Purity or Quality Grade: ≥97%