MilliporeSigma™ Leptin, Mouse, Recombinant Protein, E. coli

Description

Recombinant, mouse leptin espressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations in the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. Suppresses insulin secretion by inhibiting activities of Ca²⁺-dependent PKC isoforms. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis.

Specifications

• Formulation: Lyophilized from a sterile filtered PBS.
• Preparation Method: Reconstitute with 20mM Tris-HCl, pH 8.0 to a final concentration of 1mg/mL.
• Purification Method: SDS-PAGE
• Quantity: 1 mg
• Source: E. coli
• Immunogen: Leptin
• Storage Requirements: Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (−20°C or −70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at −20°C or −70°C. Avoid freeze/thaw cycles of solutions.
• Regulatory Status: RUO
• Species: Mouse
• Recombinant: Recombinant
• Form: Lyophilized
• Purity or Quality Grade: ≥97%