MilliporeSigma™ Upstate™ LSD1 (KDM1A), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 1710531

Catalog No. 17-105-31MI

Description

Specifically detects LSD1 (KDM1A) Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Immunoprecipitation, Western Blotting

LSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Specifications

• Antigen: LSD1 (KDM1A)
• Classification: Polyclonal
• Formulation: Anti-LSD1 (Rabbit Polyclonal). One vial containing 50μg affinity purified rabbit polyclonal in 72μL buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide, before the addition of glycerol to 30%. Final concentration of antibody 0.7mg/mL. Normal rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSA. One vial containing 75μL of 5μM of each primer specific for human prostate-specific antigen (PSA) enhancer region (chr19:51354179-51354263, hg19 build). FOR: GCC TGG ATC TGA GAG AGA TAT CAT C; REV: ACA CCT TTT TTT TTC TGG ATT GTT G
• Gene Symbols: KDM1A, AOF2, KDM1, LSD1
• Immunogen: Histidine-tagged recombinant protein corresponding to full length LSD1.
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_055828
• Target Species: Human, Mouse, Rat

• Applications: ChIP Assay, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: O60341
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Includes: Use ChIPAb+ LSD1 (KDM1A) ChIP Validated mouse monoclonal antibody for the detection of LSD1 (Lysine-specific histone demethylase 1A) also known as BRAF35-HDAC complex protein BHC110, includes the antibody and the specific control PCR primers.
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only