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MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates 
Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns
$221.00 - $438.00
| Catalog Number | Mfr. No. | Product Type | Quantity | Sensitivity | Price | Quantity & Availability | |||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Product Type | Quantity | Sensitivity | Price | Quantity & Availability | |||
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WBLUC0500
|
MilliporeSigma™
WBLUC0500 |
Luminata Classico Western HRP Substrate | 500 mL | ∽6 pg |
Each for $221.00
|
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WBLUF0500
|
MilliporeSigma™
WBLUF0500 |
Luminata Forte Western HRP Substrate | 500 mL | ∽400 fg |
Each for $438.00
|
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WBLUR0500
|
MilliporeSigma™
WBLUR0500 |
Luminata Crescendo Western HRP Substrate | 500 mL | ∽1 to 3 pg |
Each for $314.00
|
|
Description
Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.
- Consistent results with less pipetting error
The stabilizers added to Luminata substrates:
- Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
- Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
- Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation
Three different sensitivities for a range of detection needs:
- Luminata Classico premixed reagent for everyday detection needs
- Luminata Crescendo premixed reagent for detection in the picogram range
- Luminata Forte premixed reagent for detection in the femtogram range
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Specifications
| Western blotting, dot/slot blotting |
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